ITGB1 anticorps

N° du produit ABIN119428

Cet anticorps Souris Monoclonal détecte spécifiquement ITGB1 dans WB, FACS et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour ITGB1 anticorps (ABIN119428)

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGB1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: 12G10

Détail du produit anti-ITGB1 anticorps

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogène: Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract. Spleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: 1/25-1/50 ELISA: 10 μg/mL. Western Blot. Note: Clone 12G10 has been reported for use in Immunoprecipitation. However, due to the nature of the epitope recognised by clone 12G10, we do not recommend this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ITGB1

Antigène: ITGB1 (Integrin beta 1 (ITGB1))

Autre désignation: CD29 / Integrin beta-1

Sujet: Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.Synonyms: FNRB, Fibronectin receptor subunit beta, ITGB1, Integrin VLA-4 subunit beta, MDF2, MSK12

ID gène: 9606

UniProt: P05556

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome